Integrated DNA Technologies PrimeTime qPCR Assay Plates

Integrated DNA Technologies (IDT) has announced the launch of PrimeTime qPCR Assay Plates for high-throughput qPCR analysis. Primers and probes for 5′-nuclease and intercalating dye assays can now be ordered in a 96-well plate format, eliminating the time-consuming transfer of primers and probes from reagent stocks and streamlining the reaction set-up. The plates are suitable for applications that generate large quantities of data, such as validation of NGS and microarray data, as well as high-throughput gene expression screening.  

Addressing the needs of each particular qPCR assay requirement, a range of primer and/or probe concentrations are available, delivered lyophilised within a 96 deep-well plate. For additional flexibility, different dye-quencher combinations can also be ordered on the same plate, extending the range of assays that can be performed during a single run. Further savings are possible once a plate layout has been designed since replicate plates can be generated at a lower total cost per reaction, making the format suited to high-throughput applications in academia and industry. 

Designing and ordering custom assays in the plate format is rapid and intuitive with the online PrimeTime qPCR assay tool, which allows users to create a master plate via simple copy and paste actions from source data. Alternatively, researchers can select from a specialised library of predesigned human, mouse or rat assays generated by IDT’s design engine. In addition to incorporating accurate T_m and secondary structure prediction data, the assay design engine takes into account the latest data from the NCBI RefSeq database to avoid off-target amplification and SNPs, thus ensuring maximum assay performance.