Invent Biotechnologies Minute Total Protein Extraction Kit for Insects

Despite significant variation in body organisation, insects all have the same general body structure. They have segmented bodies divided into three regions: head, thorax and abdomen. The body segments are protected by a hard exoskeleton or cuticles.

From a protein extraction point of view, the unique structure of the exoskeleton makes it hard to homogenise. It is also difficult to lyse cells protected by cuticle for total protein extraction. The traditional solution-based protein extraction method such as RIPA is inefficient and protein yield is low; the profile of extracted protein using the traditional method is usually incomplete.

The Invent Biotechnologies Minute Total Protein Extraction Kit for Insects provides an efficient method for total protein extraction from insects by a combination of mechanical extraction and chemical lysis. The cell lysis buffers used are designed to be much stronger than RIPA buffer.

The kit features a simple and rapid single tube protocol and optimised buffers for insect tissues. Researchers will have the option to choose either denaturing cell lysis buffer or native cell lysis buffer, which are specifically tailored for insects. The whole procedure takes less than 10 min to complete, and the protein yield is in the range of 1–3 mg/mL. The materials provided are sufficient for 50 extractions.