One BioMed X8 HMW DNA Cartridge Kits

One BioMed has launched its X8 HMW DNA Cartridge Kits for the automated extraction of high molecular weight DNA for use in long-read sequencing and genome assembly. Inclusive of all the necessary reagents, the cartridge kits, powered by the OneTouch Instrument, provide an out-of-the-box, automated platform for extraction of HMW DNA from a myriad of sample types like bacteria, plants, cell culture, tissue and peripheral blood mononuclear cells (PBMCs).

Long-read sequencing is a third-generation technology that sheds light on certain ‘blind spots’ seen in short read sequencing methods, such as the detection of complex structural variants. However, long-read sequencing requires isolation of high-quality, HMW DNA which can be challenging to obtain. Most commercial extraction technologies compromise the quality and length of the extracted DNA, as they include mechanical steps like centrifugation or vortexing, and/or alcohol precipitation in their labour-intensive workflows. One BioMed’s patented Xceler8 Technology uses a gentle yet rapid chemistry-based method to selectively bind, gently cluster and purify intact DNA using controlled fluidics. The specially formulated workflow is also non-volatile and ethanol-free, enabling hassle-free shipping and storage at room temperature.

The Xceler8 Platform provides consistent and reproducible extraction of HMW DNA up to 2 Mb range from various sample types across different users. The purified HMW DNA is suitable for various downstream applications including long-read sequencing and de novo genome assembly. With the flexibility of random access, the technology also has the ability to run different protocols simultaneously and can efficiently process up to eight samples in as little as 1 h. The fully integrated, plug-and-play automation system is suitable for labs looking for flexible and consistent HMW DNA extraction from a variety of sample types.