PCR Biosystems NGSBIO Library Quant Kit for Illumina

PCR Biosystems has expanded its range of specialist molecular biology kits with the NGSBIO Library Quant Kit for Illumina. The kit contains all the components necessary for sensitive quantification of DNA libraries prior to next-generation sequencing (NGS) with Illumina platforms.

Employed for the vast majority of NGS, the Illumina system requires quantification of libraries prior to sequencing to ensure the correct amount of library is loaded into the machine. This is essential for generating the maximum amount of clustering data possible per run. An easy-to-see blue version of the kit has also been launched, containing a blue qPCR mix for greater pipetting precision.

The kit offers consistent quantification across a wide range of sample types, concentrations, fragment sizes and GC content. It contains five DNA standards, primers specific to the P5 and P7 Illumina adapter sequences, a convenient library dilution buffer and qPCRBIO SyGreen Mix or qPCRBIO SyGreen Blue Mix. The blue mix contains a non-reactive dye to improve reaction mix visibility and is particularly useful when using small reaction volumes or white plates.

PCR Biosystems also provides a free online calculation tool to help scientists quickly and easily analyse results generated with the kit. Users can download the results in a PDF or email the results to themselves, with the additional benefit of accessing the data online for up to 30 days.

The NGSBIO Library Quant Kit is designed to be convenient and easy to use, with the use of qPCR said to ensure optimal cluster densities for improved sequencing efficiency and data quality. qPCR offers advantages over other methods as it only measures adapter-bound molecules that can be used as templates for library amplification and cluster generation, as opposed to the quantity of all double-stranded DNA that is present.