PerkinElmer Opal Multiplex Tissue Staining
Understanding cancer biology and assessment of complex biological function requires simultaneous interrogation of multiple pathways combined with context provided by tissue images. Standard methods provide an incomplete picture: flow cytometry excels at multiplex analysis but destroys the context provided by tissue, and analysis of individual markers in serial sections is unable to promote understanding of single cell co-expression, particularly for three or more markers at a time.
With Opal Multiplex Tissue Staining, users can overcome these challenges and achieve robust, specific multiplex tissue biomarker measurement. Antibodies can be selected based on performance rather than species. The typical 4-plex Opal staining procedure may be completed within one day. The method is compatible with the standard research IHC workflow in the lab and is amenable to automation.
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