Promega MethylEdge Bisulfite Conversion System for DNA methylation analysis
The MethylEdge Bisulfite Conversion System offers an efficient method for rapidly performing bisulfite conversion and DNA clean-up within 2 h while maintaining DNA integrity to provide more intact, converted DNA for a variety of sensitive applications.
The system requires only small amounts of input genomic DNA (100 pg-2 µg). Using optimised conversion reagents, clean-up and recovery methods, the system converts DNA at ≥90% efficiency with minimal fragmentation. The resulting DNA is suitable for use in downstream applications such as PCR, array or sequencing assays.
The kit includes all reagents necessary to perform 50 bisulfite conversion reactions. The reagents can be stored at room temperature and require minimal up-front preparation. Control DNAs are also available separately and can be used as standards in both the conversion reaction and post-conversion analysis.
The product complements Promega nucleic acid purification kits for a wide variety of sample types and throughput levels, quantitation chemistries for both pre- and post-conversion, and Promega amplification technologies for detection, providing a quality workflow solution to DNA methylation sample preparation.
Phone: 03 9818 1466
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