The Qiagen Multiplex PCR Plus Kit has been designed for rapid and sensitive multiplex PCR that allows researchers to amplify several targets in one PCR run. The kit provides fast reaction times and comes with a preoptimised protocol, eliminating the need for optimisation and delivering reliable results on the first attempt.
The master mix included with the kit contains the enzyme HotStarTaq Plus DNA Polymerase, as well as a balanced combination of salts and additives, which increases multiplex reaction specificity and ensures comparable efficiencies for all primers in the PCR reaction. This technology allows straightforward assay development for many challenging applications, such as detection of mutations or microsatellites and microbial genotyping.
The kit also includes CoralLoad - a buffer containing two dyes that improves pipetting visibility during reaction set-up, gel loading and visualisation of DNA migration. Therefore, it is no longer necessary to use other dyes for gel electrophoresis.
Downstream analysis of the multiplex PCR products can be performed with a variety of methods such as agarose gels, capillary sequencers, as well as multicapillary electrophoresis with the QIAxcel Advanced System.
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