Qiagen has launched five additions to its EpiTect product line to help address the growing demand for standardised products for complete workflows in epigenetics.
The EpiTect Whole Bisulfitome kit is a solution for reliable and convenient amplification of the entire bisulfilte-converted genome. The kit incorporates REPLI-g technology and is adapted to smaller DNA fragment size and modified nucleotide composition resulting from bisulfite conversion.
The EpiTect MSP kit includes a genetically engineered DNA polymerase for end-point methylation-specific PCR (MSP). This provides highly specific discrimination between methylated and unmethylated cytosine after bisulfite conversion. False-positive results are thus reduced.
The EpiTect MethyLight PCR kit is a sensitive kit for quantitative analysis of methylated DNA, using probe-based real-time PCR. Using just one sample, this product enables the quantification and differentiation of even small variations in methylated and unmethylated DNA.
The EpiTect MethyLight Assay probe-based real-time assays enable the standardised quantification and differentiation of methylated and unmethylated DNA for six selected genes related to cancer.
Finally, the EpiTect Control DNA is a ready-to-use, bisulfite-converted DNA for highly reliable methylation-control experiments.
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