Thermo Fisher Scientific direct PCR approach

The Direct PCR approach delivers convenience for DNA amplification by allowing PCR to be performed directly on unpurified samples. Eliminating the DNA purification step allows significant savings in both time and cost. In addition, using only a tiny amount of source material (including blood, animal, plant and human tissue) means that precious samples can be saved for other applications.

Direct PCR is based on the company’s Phusion High-Fidelity DNA Polymerases and Phire Hot Start II DNA Polymerase. These fusion enzymes have been engineered to incorporate a double-stranded DNA binding domain that gives them desirable properties. Phusion and Phire DNA Polymerases are tolerant of many PCR inhibitors present in various sample material. This allows robust amplification in conditions where conventional DNA polymerases are completely inhibited.