Thermo Fisher Scientific Invitrogen TaqMan and SYBR Green Fast Advanced Cells-to-CT Kits

Thermo Fisher Scientific has launched its Invitrogen TaqMan and SYBR Green Fast Advanced Cells-to-CT Kits. Users will experience a fast reverse transcriptase reaction and have access to a qPCR master mix system fully optimised to improve sensitivity to detect rare transcripts. This makes it possible to perform high-throughput expression analysis directly from cultured cells without RNA purification and without risking sensitivity.

The product offer researchers fast workflows and high sensitivity and detection of abundant and rare transcripts. The kits help save time and offer a simple workflow that is suitable for a few samples, but can also be easily incorporated into automated, high-throughput applications. Researchers can focus on higher value activities, such as analysing high-throughput drug screening data or performing supplementary genome editing or silencing validation studies.

The kits employ a 5 min lysis system to quickly lyse cultured cells while simultaneously removing genomic DNA (gDNA) and preserving RNA integrity. The Cells-to-CT Stop Solution irreversibly terminates the lysis reaction, enabling users to carry the most sample into the RT and qPCR reactions to maximise the detection of RNA transcripts.

Detecting rare RNA transcripts in low-input cell samples (10–1000) is said to be higher when samples are processed with Cells-to-CT kits compared to results from the same samples using RNA purification workflows. The kits are fully optimised with updated reverse transcription (RT) reagents for cDNA synthesis and fast TaqMan or SYBR Green master mixes for real-time PCR analysis.