Thermo Scientific SMART Digest protein analysis kit

The Thermo Scientific SMART Digest protein digestion kit is designed to reduce preparation times and associated errors, facilitating fast analytical results for biopharmaceutical and protein research applications.

Current protein digestion techniques comprise complex procedures that require the determination of protein concentration, followed by sample reduction and alkylation steps prior to digestion with trypsin. This multifaceted preparation protocol can take up to 24 h and is prone to both irreproducibility and poor sensitivity.

To improve on this process, the Thermo Scientific SMART Digest kit uses a three-step, easy-to-implement process that can be automated. Its immobilised trypsin design is formulated for high-quality data and is said to have a reduced sample preparation time compared to traditional in-solution digestion methods.

The increased reproducibility of digestion possible with the kit should enable users to implement generic, fast and robust methods for high-throughput processing of samples. Furthermore, the kit is designed to provide high sequence coverage while reducing both chemically induced post-translational modifications and the number of chemicals in the final sample. This design makes the kit accurate and sensitive, capable of generating high-quality data at high throughputs.