3D genome reconstructed from freeze-dried woolly mammoth

An international research team has assembled a 3D reconstruction of the genome and chromosomal structures of a 52,000-year-old woolly mammoth, in what is understood to be a world first for any ancient DNA sample.

As documented in the journal Cell, the fossilised chromosomes provide insight into how the mammoth’s genome was organised within its living cells and which genes were active within the skin tissue from which the DNA was extracted. This unprecedented level of structural detail was retained because the mammoth underwent freeze-drying shortly after it died, which meant that its DNA was preserved in a glass-like state.

“This is a new type of fossil, and its scale dwarfs that of individual ancient DNA fragments — a million times more sequence,” said corresponding author Erez Lieberman Aiden, Director of The Center for Genome Architecture at Baylor College of Medicine. “It is also the first time a karyotype of any sort has been determined for an ancient sample.”

Knowing the three-dimensional architecture of a genome provides a lot of additional information beyond its sequence, but most ancient DNA specimens consist of very small, scrambled DNA fragments. Building off work mapping the 3D structure of the human genome, Aiden thought that if the right ancient DNA sample could be found — one with the 3D organisation of the fragments still intact — it would be possible to use the same strategies to assemble ancient genomes.

The researchers tested dozens of samples over the course of five years before landing on an unusually well-preserved woolly mammoth that was excavated in north-eastern Siberia in 2018. According to corresponding author Olga Dudchenko, also from The Center for Genome Architecture, “We think it spontaneously freeze-dried shortly after its death. The nuclear architecture in a dehydrated sample can survive for an incredibly long period of time.”

To reconstruct the mammoth’s genomic architecture, the researchers extracted DNA from a skin sample taken behind the mammoth’s ear. They used a method called Hi-C that allows them to detect which sections of DNA are likely to be in close spatial proximity and interact with each other in their natural state in the nucleus.

“Imagine you have a puzzle that has three billion pieces, but you don’t have the picture of the final puzzle to work from,” explained corresponding author Marc A Marti-Renom, an ICREA research professor and structural genomicist at the Centre Nacional d’Anàlisi Genòmica (CNAG) and the Centre for Genomic Regulation (CRG) in Barcelona. “Hi-C allows you to have an approximation of that picture before you start putting the puzzle pieces together.”

Examination of the skin sample from the 52,000-year-old woolly mammoth. Image credit: Love Dalén, Stockholm University.

Then, they combined the physical information from the Hi-C analysis with DNA sequencing to identify the interacting DNA sections and create an ordered map of the mammoth’s genome, using the genomes of present-day elephants as a template. The analysis revealed that woolly mammoths had 28 chromosomes — the same number as present-day Asian and African elephants. Remarkably, the fossilised mammoth chromosomes also retained a huge amount of physical integrity and detail, including the nanoscale loops that bring transcription factors in contact with the genes they control.

By examining the compartmentalisation of genes within the nucleus, the researchers were able to identify genes that were active and inactive within the mammoth’s skin cells — a proxy for epigenetics or transcriptomics. The mammoth skin cells had distinct gene activation patterns compared to the skin cells of its closest relative, the Asian elephant, including for genes potentially related to its wooliness and cold tolerance.

“For the first time, we have a woolly mammoth tissue for which we know roughly which genes were switched on and which genes were off,” Marti-Renom said. “This is an extraordinary new type of data, and it’s the first measure of cell-specific gene activity of the genes in any ancient DNA sample.”

Although the method used in this study hinges on unusually well-preserved fossils, the researchers are optimistic that it could be used to study other ancient DNA specimens —from mammoths to Egyptian mummies — as well as more recently preserved museum specimens. For mammoths, the next steps would include examining the epigenetic patterns of other tissues.

“These results have obvious consequences for contemporary efforts aimed at woolly mammoth de-extinction,” said corresponding author M Thomas Gilbert, a paleo-genomicist at the University of Copenhagen and the Norwegian University of Science and Technology.

Top image credit: iStock.com/Aunt_Spray