Brain cells in Alzheimer's patients consume amino acids

Alzheimer’s disease (AD) causes the death of neurons and leads to shrinking of the brain, with patients experiencing a gradual deterioration in memory, thinking, behaviour and the ability to perform everyday activities. Hong Kong researchers have now suggested that early changes in brain metabolism may explain this neurodegeneration.

The research team, led by The Chinese University of Hong Kong (CUHK), discovered that a special gene variant causes astrocytes of the brain to exhaust the primary substance for neurotransmission: amino acids. The long-term loss of amino acids may result in compromised neurotransmission and hence cognitive and memory impairments. The team’s findings, published in the journal Advanced Science, also indicate that direct brain supplement of the missing amino acids was effective in alleviating the neurodegenerative outcomes as well as the functional degeneration.

While the predominant approach towards AD treatments has targeted beta-amyloid — the classic pathology of AD — the repeated failures of clinical trials of anti-amyloid therapies highlight the pressing need to identify novel molecular targets that may underlie AD pathogenesis. Recently, a growing body of evidence has hinted that AD is a pervasive metabolic disorder in which altered cellular fuel metabolism occurs at the early prodromal stages of the disease long before the symptoms manifest. Therefore, understanding how perturbations in metabolism are related to this prodromal stage is critical to identifying targets for disease-modifying therapies.

A previous study suggested an association between a synonymous single nucleotide polymorphism (SNP) in LRP6 gene and AD. In the latest study, the team found that this SNP is indeed correlated with diminished LRP6 gene expression in the forebrain region and the gene is predominantly expressed in astrocytes — the metabolic workhorses in the brain. By means of genetic deletion of LRP6 specifically in these cells within the adult forebrain of mice, both their cognitive and memory function were dramatically impaired. This phenotype is associated with extensive metabolic reprogramming occurring in the brain microenvironment.

“Findings show that the correct partitioning of fates of various metabolites is critical for maintaining the normal synaptic function in the brain,” explained CUHK Professor Kim Hei-Man Chow, principal investigator of the study.

“In normal situations, LRP6 drives a metabolic program which facilitates astrocytes to utilise glucose as its major fuel source for energy production. In the absence of LRP6 function, the entire metabolic landscape in astrocytes is rewired, rendering them unable to utilise glucose but [rather] glutamine, an amino acid which is also a neurotransmitter released by neurons, together with branched chain amino acids (BCAAs) as fuel. This shift in metabolic dependence not only resulted in impaired brain glycolytic capacities but also exhausted amino acids that are essential for neurotransmitter recycling and synthesis. When this situation was manifested chronically, this consequently hampered synaptic fidelity, which is the molecular basis of normal cognitive and memory functions.”

The metabolic changes not only affect people who harbour the gene polymorphism at LRP6 locus, they are also relevant to those with the genetic variant of the apolipoprotein E (APOE) E4 allele, the most common genetic risk factor of AD. The research team found that protein product of the APOE-E4 allele hampers the normal functioning of LRP6 protein by trapping it inside the cell, keeping it away from the cell surface where it carries out its functions.

With recent advances in the field suggesting that brain metabolic dysfunction is at the core of Alzheimer’s disease, targeting the new metabolic need of the brain may become a potential strategy to manage the disease progression. The research team revealed that direct brain supplement of the missing glutamine and BCAAs was effective in alleviating the neurodegenerative outcomes as well as the functional degeneration.

“Specific nutrient supplementation is nowhere a new concept in clinics,” Prof Chow said. “Glutamine is supplemented clinically to patients with critical illness. On the other hand, all BCAAs are by default essential amino acids, and their oral supplements have been used with good tolerance in treatments of several neurologic diseases, including bipolar disorder, tardive dyskinesia, amyotrophic lateral sclerosis and spinocerebellar degeneration. These clinical applications indicated that both glutamine and BCAAs can be consumed in considerable amounts by humans without adverse effects, and in many cases, with significant benefits.

“Together, our findings support that, in addition to traditional drug-based therapies, the adjunct nutrient-based approach has great potential for managing neurodegenerative disorders.”

Image credit: ©stock.adobe.com/au/Andrea Danti

Please follow us and share on Twitter and Facebook. You can also subscribe for FREE to our weekly newsletters and bimonthly magazine.