Live cell imaging with Opera

Opera from Evotec Technologies is a confocal microplate imaging reader offering a technically advanced set of solutions for fully automated high speed and high resolution cellular screening.

Significant barriers to high-throughput live cell imaging have now been overcome on the new generation Opera with the addition of precision environmental controls.

Maintaining live cells at or near physiological conditions is a challenging but essential requirement for the success of many screening programs.

On the new Climate Control Opera, temperature, CO2 and humidity levels are easily set and precisely maintained. A chamber enclosing the entire plate receptacle and lens area eliminates any temperature gradients that might arise from contact with instrument components.

Potential cell stress is reduced even further by the use of Nipkow spinning disk imaging technology, which minimises photo toxicity while enabling high speed confocal imaging.

Together, these new technologies make high-throughput live cell imaging both practical and reliable. Additional enhancements to the new Climate Control Opera system include a dispensing unit, which may be integrated for kinetic experiments.

The Opera serves the ever-increasing demand in drug discovery and high throughput biology. It has four lasers (405 nm, 488 nm, 532 nm, 635 nm) plus a high pressure Xenon lamp available for fluorescence excitation in the range from 360 nm to 635 nm.

Images can be acquired by up to four CCD cameras in parallel, enabling high speed multi colour image acquisition and analysis, as well as FRET applications.

Up to six bandpass filters per camera make it possible to optimally match acquisition settings to the application.

PerkinElmer Life and Analytical Sciences

Tel: 1800 033 391

www.perkinelmer.com