Scientists find new strains of influenza A virus in pigs

Scientists from Duke-NUS Medical School and their collaborators have uncovered several previously unknown strains of swine flu viruses that have been circulating unnoticed in Cambodian pig populations over the past 15 years, potentially posing a pandemic risk.

Described in the journal PNAS, the strains include viruses that have been passed by humans to pigs, as well as some with genes originating from as far as North America. The study makes the case for systematic surveillance to detect and warn of new strains of viruses early to prevent future pandemics.

Pigs are a key intermediary in the emergence and potential spread of influenza viruses between animals and humans, the researchers noted, as they provide a suitable environment for the shuffling of gene segments between avian, swine and human hosts, ultimately giving rise to new viruses. With pork production dramatically increasing over the past 50 years, international trade and movement have further amplified the risks.

“The long-term evolution of different lineages has led to the establishment of genetically distinct viruses that have been continuously circulating in pig populations undetected for decades,” said Duke-NUS’s Professor Yvonne Su, a senior and corresponding author of the study. “Our study revealed the hidden and complex genomic landscape of swine flu virus evolution in South-East Asia, marking the region as a hotspot for virus diversity and risk of new virus emergence.”

From March 2020 to July 2022, Su and her collaborators conducted swine influenza surveillance in 18 pig slaughterhouses in Cambodia. They collected 4089 nasal swabs from pigs in different districts of four provinces. Among these, 72 pigs — or around 2% — tested positive for influenza A virus.

The scientists identified nine distinct swine influenza A virus groups, at least seven of which had not been detected for 2–15 years. Among these are multiple H3 lineages that had been passed by humans to pigs, circulating undetected for about 10 years; as well as the H1N1 subtype, which was predominant and likely derived from human origins dating back to the 2009 pandemic. Two seasonal viruses were detected in pigs from Kandal, Phnom Penh and Takeo provinces, and likely originated from Thailand. The team also isolated a new swine European H1N2 variant (that originally came from birds) with North American genes in Cambodia. While they were the first to detect this variant, their genomic analysis suggests that it had been circulating in pigs in the region since 2014, highlighting the need for better surveillance.

Delving deeper into the movement of viruses across geographical borders, the scientists found that European swine flu viruses had been sporadically introduced into South Central China and South-East Asia in the early 2000s. Genetic evidence indicated South Central China has served as the major source of European-like swine flu virus transmission in the region since around 2010, with the viruses subsequently spreading more widely across China and South-East Asian countries such as Cambodia.

“While swine influenza viruses typically cause mild symptoms in pigs, they pose a pandemic threat to humans, as the human population may lack immunity or have inadequate protection against new strains of swine influenza viruses,” said co-author Professor Gavin Smith, Director of Duke-NUS’s Emerging Infectious Diseases program. “Therefore, systematic surveillance is crucial in early detection and warning of new subtypes or strains.”

Further studies are needed to understand the pandemic threat of the new viruses, including how they react with human viruses and how easily they can spread. To this end, the team is currently developing a platform that can identify major swine flu genetic subtypes, including avian sequences. With this set-up, they will be able to assess if pig and human populations have been infected with the influenza subtypes.

“Routine and sustained surveillance is indispensable in identifying new viruses so that their transmission risk can be assessed,” said Professor Patrick Tan, Senior Vice-Dean for Research at Duke-NUS. “It is therefore critical that more efficient and continuous surveillance methods are integrated with automated analytical tools to rapidly provide information on changes in human and animal pathogens. Such a system as the team at Duke-NUS is developing would improve animal health through selection of effective vaccines, and aid in human health by monitoring viruses with the potential for transmission.”

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