Rapid and thorough DNA, RNA and protein extraction

By MP Biomedicals Australasia P/L
Thursday, 18 September, 2014 | Supplied by: MP Biomedicals Australasia P/L

Rapid and thorough DNA, RNA and protein extraction

The FastPrep-24 5G high-speed benchtop homogeniser is an ultrahigh-performance sample preparation system that allows for the extraction of fully intact, biologically functional macromolecules from routine as well as highly resistant samples.

The high-speed benchtop homogeniser can be used for grinding, lysing or homogenising - facilitating the easy and reproducible isolation of stable RNA, active proteins and full-length genomic DNA. Applications include but are not limited to all types of human, animal and plant tissues including cultured cells; bacterial, yeast and fungal cells, including spores and oocytes; environmental and metagenomic samples including soil and faecal samples; and other inorganic solid matrices.

The intuitive software, microprocessor control and high-definition touch screen programming features in the FastPrep-24 5G ensure that optimisation time is minimised so users have more time to analyse data.

The FastPrep-24 5G uses a unique, optimised motion to disrupt cells through the multidirectional, simultaneous beating of specialised Lysing Matrix beads on the sample material. Samples and buffers are added to Lysing Matrix Tubes containing the beads, supplied ready to use, certified nuclease-free and in a variety of sample type specific compositions.

The instrument lyses thoroughly and quickly any tissues and cells and thus allows easy and reproducible isolation of stable RNA, active proteins and full-length genomic DNA.

The sample tubes remain securely sealed during the processing and the single-use design eliminates cross-contamination. Program parameters are easily set using the touch screen user interface, or users can choose from the 70-plus recommended programs,  user-defined saved programs or user-defined custom programs stored on the 5G’s onboard computer.

The recommended programs are the heart of the 5G’s functionality. These validated programs include all variable assay parameters. This is a valuable optimisation tool for new users and is of special interest to those who are working with pathogenic or dangerous samples, as well as low abundance samples.

FastPrep-24 uses a unique, optimised motion to disrupt cells through the multidirectional, simultaneous beating of specialised lysing matrix beads on the sample material. FastPrep will homogenise up to 24 samples in 2 mL tubes or, with optional adapters, lyse 48 samples in 2 mL tubes, 24 samples in 4.5 mL tubes, 12 samples in 15 mL tubes or 2 samples in 50 mL tubes making FastPrep a particularly versatile homogeniser. Developed for difficult and resistant samples, FastPrep-24 thoroughly and quickly lyses all tissues and cells providing easy and reproducible isolation of stable RNA, active proteins and full-length genomic DNA.

  • Powerful: The highest speed available provides the thorough grinding, homogenising and lyses of the most difficult samples in just a few seconds.
  • Highest yield and purity: The most DNA, RNA and proteins from any sample type including the most resistant samples. FastPrep-24 5G with FastPrep Kits provide the highest yield and purity. Programmed protocols.
  • Intuitive: Interactive user-friendly interface and touch screen for easy programming and numerous, >70, recommended pre-programmed protocols for a large variety of applications.
  • Complete solution: Largest number of ready-to-use Lysing Matrix compositions and FastPrep Kits for DNA, RNA and protein purification of any sample and tissue application.
  • Flexible: Easily interchangeable adapters to process any sample size (2, 4.5, 15 or 50 mL tubes) at cryogenic or room temperature. Up to 48 samples can be processed at one time under ambient or cryogenic conditions.
  • The NextGen Sample Prep delivers the most DNA, RNA and proteins from the most resistant samples in 40 seconds or less.

MP Biomedicals Australasia P/L
www.mpbio.com

Online: www.mpbio.com/au/
Phone: +61 2 8824 2100
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