Chemical cues turn embryonic stem cells into cerebellar neurons

In order to differentiate and specialise, stem cells require very specific environmental cues in a very specific order, and scientists have so far been unable to prod them to go through each of the necessary steps.

For the first time, Rockefeller University scientists have shown in a mouse study that embryonic stem cells implanted in the brain appear to develop into fully differentiated granule neurons, the most plentiful neuron in the cerebellum.

The findings were reported in the February 20 online edition of Proceedings of the National Academy of Sciences.

The cerebellum contains neural circuits that are responsible for motor learning, motor memory and sensory perception. It's also the location of 40 per cent of paediatric brain tumours. Rockefeller's Professor Mary E. Hatten, who has been studying granule cells for 30 years, sees her results as a step toward understanding how embryonic stem cells could be regulated in vivo and ultimately used for cell replacement therapy, especially after childhood tumours, in the central nervous system.

Hatten and postdoc Enrique Salero found that in order to get the embryonic stem cells to differentiate, progressing through each of the known steps of granule neuron maturation as they did so, the cells had to be treated with signals that induce specific transcription factors in a specific order. The researchers then implanted the newly differentiated cells into the cerebellar cortex of newborn mice.

Once in the brain, the cells extended parallel fibres, migrated to and incorporated themselves into the internal granule cell layer and into dendrites. Each of these steps, Hatten says, is characteristic of a typical granule cell.

Salero and Hatten then looked for evidence that their embryonic stem cells had not just gone through the developmental steps of young granule neurons, but that they also had the known markers of young granule neurons, including those indicating that the neurons had formed in the cerebellum.

"We're excited about this paper because it's the first time that anybody has shown that a cell not only migrates to where it's supposed to go, but extends dendrites," Hatten says. "So they're actually in the synaptic network that's sitting on the cortex."

Hatten isn't yet convinced that the cells differentiated into true granule neurons. "There is such wild-eyed enthusiasm over stem cells but it's very hard to know when you've provided sufficient evidence that a cell is actually what you say it is."

So her next step will be to work with Nathaniel Heintz, an HHMI investigator, to determine how close a genetic match the native granule cells are to the embryonic stem cell-derived versions.

"This whole field of stem cell biology is exciting, but also frightening because of the potential harm that could be done," Hatten says. "We have made a lot of progress with stem cells outside the brain, especially with the heart and skin.

"But neurons in the brain seem to undergo more complicated genetic changes as they progress through a long series of maturation steps. So we want to be absolutely sure that we're generating neurons that will aid, rather than hamper, brain function."

Source: Rockefeller University