Key protein assists in 'streamlining' sperm for travel

In order to achieve good times in their races, many Olympic swimmers wear swimsuits that are low in water resistance. Similarly, spermatozoa, the male reproductive cells, possess a ‘streamlined’ structure to travel more smoothly through the female reproductive tract. Now Japanese researchers have shed new light on a key protein involved in this process, which they have revealed in the journal PNAS.

During reproduction, spermatozoa must travel through the female reproductive tract to reach the oocyte, or egg, in order for fertilisation to occur. To better facilitate this process, spermatozoa have a ‘streamlined morphology’ that is achieved by eliminating sperm cytoplasm. While this process has been observed in previous studies, the molecular mechanisms underlying it are not fully understood. This led researchers from Osaka University to explore a mouse model targeting a protein known as testis-specific serine kinase substrate (TSKS), which is localised to membraneless structures called nuage.

“Using genome editing technology, we developed a mouse model in which TSKS has been disrupted,” said Keisuke Shimada, co-lead author of the study. “We found that spermatozoa from the mice with disrupted TSKS failed to develop a streamlined form, resulting in male infertility.”

The researchers analysed the spermatozoa from TSKS knockout mice and found that these sperm were unable to produce two specific types of nuage called reticulated body (RB) and chromatid body remnant (CR). Without these nuage, the TSKS-disrupted sperm could not properly eliminate their cytoplasm. Additionally, the researchers observed that the presence of excess residual cytoplasm led to apoptosis, or cell death, in these spermatozoa.

“Our results showed that generation of RB and CR nuage is dependent on TSKS,” said co-lead author Soojin Park. “TSKS is required for sperm to eliminate cytoplasm and adapt a streamlined, tadpole shape. This applies to humans as well, as TSKS is also present in human spermatozoa.”

This discovery of the role of TSKS on the formation of streamlined spermatozoa offers more clues on one mechanism behind male infertility. The findings from this study may therefore translate to the development of diagnostic tests and male contraceptives.

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