IDT Custom NGS Adapters

Integrated DNA Technologies (IDT) has launched its Custom NGS Adapters offering, providing an easy and flexible customised adapter solution directly to scientists. The cornerstone of the offering is an innovative, web-based configurator tool — the Custom Adapter Configurator — enabling researchers to select exactly the right components for their experiments and immediately order them using a built-in e-commerce function. Completing the line-up is the Lotus DNA Library Prep Kit, providing a simple, robust and flexible library preparation.

NGS adapters are a specific type of custom oligo. Unlike other array and chip-based manufacturers, IDT adapters and other products are individually synthesised using stringent, proprietary methods that deliver high quality in every batch — critical for NGS applications. IDT has now made its NGS adapters easily accessible to users of all levels, enabling researchers to tailor library preparation to their specific applications. There are three configurations available — TruSeq-Compatible Full-length Adapters, TruSeq-Compatible Stubby Adapter and Indexing Primers, and Nextera-Compatible Indexing Primers.

Accompanying the adapter selection is the guided Custom Adapter Configurator online tool, taking the difficulty and complexity out of adapter selection and making it easy for researchers to identify and purchase exactly what they need in a matter of minutes. The tool provides expert guidance, effortlessly walking the user through otherwise complex adapter design and formatting options.

Completing the portfolio expansion is the Lotus DNA Library Prep Kit — a rapid, easy and flexible enzymatic library preparation solution for a multitude of whole genome sequencing (WGS) and hybridisation capture applications. With a 2 h workflow, the kit — optimised for use with IDT’s custom adapters and xGen capture products — delivers good coverage and uniformity for sequencing on Illumina platforms. IDT has designed the kit with flexibility in mind, enabling it to be compatible with TA-ligation adapters and tailored to almost any application when combined with the adapter options.